T Using your straw, GENTLY push the Elodea to the bottom of the test tube. Thus, the more photosynthetic activity, the less acidic and more blue the bromothymol blue solution becomes as the plant uses carbon dioxide during photosynthesis. Okay so here are the details Beaker 1 was added bromothymol blue indicator and I blew on the water which change the color to yellow, then I added elodea plant. 9. 2. Purpose: In this lab you will observe the interaction of a snail and a water plant in a closed environment. Photosynthesis lab with elodea and bromothymol blue >>> click to continue How to write a good acceptance essay Write my english essay font my code heading pay someone who wants to buy swot analysis essay teacher arial smart custom writing help,. Place 90 mL of distilled water into a 150-mL beaker. Tube 1 Tube 2 Tube 3 No Elodea Elodea Elodea + + + Covered in tinfoil Covered in tinfoil In the light Pre-Lab Questions: Predict what will happen to the color of the solution when you blow into the straw (causing the bromothymol blue + water solution to bubble). Write the equation for photosynthesis: Photosynthesis occurs in the chloroplast, an organelle in plant cells that contains the molecule chlorophyll. Can be used to teach plant leaf morphology. When the pH is above a 7 (basic) it is blue, but when the pH drops below 7 (acidic) it starts to turn yellow. Elodea High School Inquiry/Scientific Method Plants. Elodea and Bromothymol Blue are often used in classrooms to demonstrate that plants use carbon dioxide during photosynthesis. Label four test tubes: "control," "3 cm elodea" and "7 cm elodea." The purpose of this lab is to determine the effects of elodea on CO 2 levels and to observe evidence of cellular respiration and photosynthesis. Bromothymol Blue (BTB) is a blue-green liquid which changes to a yellow color in acid and back to blue-green when returned to a neutral pH. Note: Bromothymol blue turns blue … Research this chemical and state why you believe it was added to the tubes in both of these experiments. More specifically, you will learn how the amount of light affects photosynthesis. A small piece of elodea will be introduced to a solution containing bromothymol blue and CO 2. This inquiry-based activity is hands-on for students and allows them to investigate photosynthesis by determining whether plants consume or release carbon dioxide. Record the color of the solution in the data table in the question section of this lab. …H b b Ò Ò ¥ šH ç" ç" ç" ™! Then cover Tube 2 with tinfoil and place it in the test tube rack. ______________________ ___________________________________________________________________________________________________________________________________________________________________________________________________________________________________________________ Predict what color Tube 1 will be after the experiment. Bromothymol blue is an acid-base indicator that turns yellow in acidic solutions and blue in alkaline solutions. (In a beaker of 200mL of water, I add about 4mL of bromothymol blue). I believe it was added because it is an indicator that can change color in the presence of carbon dioxide and acts as an indicator of photosynthesis. W An introduction to photosynthesis using Bromothymol blue and elodea. e Elodea and Bromothymol Blue. pH = 6).This occurs when the concentration of CO 2 is high. This allows the students to focus on only one process -- either photosynthesis or respiration. ° Remove several leaves from around the cut end of the stem. The point is to determine whether the elodea … Put twenty-four drops of bromothymol blue into the beaker. Place 90 mL of distilled water into a 150-mL beaker. What happened in the test tube with only Elodea under light? Demonstrate that environmental CO2 is used during photosynthesis in Elodea. More specifically, you will learn how the amount of light affects photosynthesis. This inquiry-based activity is hands-on for students and allows them to investigate photosynthesis by determining whether plants consume or release carbon dioxide. When carbon dioxide is present the color will change from blue to yellow. Image below is courtesy PureySmart on Wikimedia Commons. This lab involves the qualitative measurement of the changes in carbon dioxide concentration associated with respiration and photosynthesis in the freshwater plant Elodea. that the photosynthesis process is slowed down until you start the experiment. ç" ™! It starts as a blue solution in water, and turns yellow in the presence of carbon dioxide. Bromothymol blue (BTB) is a pH indicator. In order to do this, you will use an Elodea plant, the chemical (lowers the pH) Bromothymol blue is an indicator solution for acids and bases. Explain your prediction. It turned blue because the plant removed all of the Carbon Dioxide from the environment What happened in the test tube with only Elodea in the dark? ™! __________________ Results: Record your results in the table below. SampleNo Elodea + Tinfoil (Control)Elodea + TinfoilElodea + LightColor Before ExperimentColor After Experiment Conclusions: Write 2 to 3 sentences based on your results. 7. Carbon dioxide bubbled into water forms carbonic acid. Elodea plant, the chemical . _________________________ _________________________________________________________________________________ What gas do you exhale? This lab involves the qualitative measurement of the changes in carbon dioxide concentration associated with respiration and photosynthesis in the freshwater plant Elodea. ™! Ø Add 2 mL of bromothymol blue to the water. Carbon dioxide has a pH of 5.7. The use of an indicator will allow you to observe the presence Materials. Fill vials #1, 2, 3 and 5 with this CO 2 enriched solution containing bromothymol blue. Bromothymol blue is an indicator solution. %���� ™! ™! The presence of carbonic acid causes the pH of a water environment to become more acidic. Using a straw, GENTLY blow into the solution causing it to bubble for approximately 1 minute. ™! __________________________________________________________________________________________________________________________________________________________________ What is the purpose of the control? Bromothymol blue works because it is able to detect the presence of CO2 (needed for photosynthesis) and O2 (released during photosynthesis) in solution. An introduction to photosynthesis using Bromothymol blue and elodea. … Before beginning the lab, I like to demonstrate to the students how bromothymol blue works. In a test tube, you will see that: bromothymol blue + CO2 = green color. <>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 612 792] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> O2 (released during photosynthesis) in solution. Bromothymol blue works because it is able to detect the presence of . 4 0 obj > ? Label 3 large test tubes: 1, 2, & 3 Tube 1 will be the control (no Elodea) Tube 2 will be the Elodea in the dark Tube 3 will be the Elodea in the light Pour 25 mL of the bromothymol blue + water solution into each of the three test tubes. 2. All of the materials are listed above, the only specifics would be that you need: 4 beakers 2 Aquarium Snails 2 Eloda bromothymol blue Fresh Water… %PDF-1.5 á Add enough bromothymol blue for the water to be visibly blue. ‡ The first set overviewing the fundamentals of photosynthesis Expose the cylinder to sunlight for 20 minutes. Bromthymol blue is used as an indicator for the presence of CO2 in solution. Regular Photosynthesis Lab - Elodea & Bromothymol Blue. If the plant uses the carbon dioxide in the water, what will happen to the amount of carbonic acid in the water? In this lab what does it indicate? Students will record their data which will be used to conclude whether carbon dioxide was consumed or released by the elodea. Abstract: This lab involves the qualitative measurement of the changes in carbon dioxide concentration associated with both respiration and photosynthesis in the fresh water plant Elodea. Photosynthesis Lab with Aquatic Plants and Freshwater Algae Overview Photosynthesis is the process by which plants transform light energy into chemical energy for growth, reproduction, and metabolism of the organism. Place a piece of elodea in the 25mL graduated cylinder with the yellow solution and insert a cork stopper in the beak of the cylinder. Bromothymol blue works because it is able to detect the presence of CO 2 (needed for photosynthesis) and O 2 (released during photosynthesis) in solution. <> Ø Put twenty-four drops of bromothymol blue into the beaker. _H aH aH aH aH aH aH $ HJ R šL Ê …H b ™! how do we know the elodea was performing photosynthesis? Students will perform two sets of experiments. Photosynthesis Lab - Elodea & Bromothymol Blue Period _____ Date _____ Background: In this lab, you will investigate the process of photosynthesis. Take all of the beakers and mix bromothymol blue with the water until you see a color change. Record your results. In order to do this, you will use an Elodea plant, the chemical Bromothymol Blue (which acts as an indicator to show if photosynthesis is occurring). 1. __________________ Predict what color Tube 2 will be after the experiment. Measure 40ml of the prepared tap water into each of their two test tubes. …H …H v v „ ú d Ñ" v v ú Biology Name ____________________________________ Photosynthesis Lab - Elodea & Bromothymol Blue Period ___________ Date ___________________ Background: In this lab, you will investigate the process of photosynthesis. –BTB reaction: Blue BTB + CO 2 → YELLOW BTB Photosynthesis Lab In Elodea Plant Elodea & Photosynthesis Photosynthesis is the process by which green plants and some other organisms use sunlight to synthesize nutrients from carbon dioxide and water Photosynthesis in plants generally involves the green pigment chlorophyll and generates oxygen as a by-product. Note: Bromothymol blue turns blue … w! " Add water to test tube and a pinch of baking soda. Place the sprig in a test tube, cut side up. Î Ï Ú ô ì `' C“ ì `' C“ ôÿ `' åÜ ä `' C“ ä `' C“ ôÿ `' åÜ Òÿ `' ê Òÿ `' ê Çÿ `' åÜ ì `' C“ ì `' C“ Çÿ `' åÜ ôÿ `' ɹ Çÿ `' åÜ ì `' C“ ì `' C“ ¸ÿ `' åÜ © `' C“ $ As a result, an acid-base indicator such as bromothymol blue can be used to indicate the presence of carbon dioxide. The absorption of Co2 from water by the elodea in light is revealed through the change in color of the bromothymol blue from blue … In a test tube, you will see that: bromothymol blue + CO2 = green color bromothymol blue + O2 = blue color Equation: Write the equation for photosynthesis. Using the eye dropper, add about 100 drops of the bromothymol blue into each of the test tubes. O. [The yellow solution will change back to blue because the plant will utilize the carbon dioxide, making the solution less acidic, converting the color back to blue. È Bromothymol blue 2 aquarium snails 2 elodea (aquarium plant) pond water 4 beakers light lamp Procedure: 1. Ÿ Picture the relationship of pH to CO 2 concentration being on a Elodea and Bromothymol Blue Photosynthesis is the process by which plants take carbon dioxide from the atmosphere, add water, and use the energy of sunlight to produce sugar. Learn vocabulary, terms, and more with flashcards, games, and other study tools. In this experiment/Lab we tested the process of Photosynthesis which included Carbon Dioxide, and living organism, water, Bromothymol Blue, and Sugar. Science fair project that examines how light wavelengths affect photosynthesis in the aquatic plant: Elodea. ___________________________________________________ _________________________________________________________________________________ Compare the Elodea in the dark to the Elodea in the light. ________________________________________________________________________ _________________________________________________________________________________ What gas (or gases) can bromothymol blue serve as an indicator for? … Oxygen has a pH of 6.7. 2. 7. __________________________________________________________________________________________________________________________________________________________________ What happened to the color in Test Tube 3? It is normally blue but turns yellow when the pH lowers. 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